AIMS--To understand the mechanism for regulation of intraocular pressure, human trabecular cells were examined to determine whether they could respond to the change in hydraulic pressure. METHODS--Human trabecular cells were cultured from trabeculum tissue fragments excised during trabeculectomy in four eyes of three patients with primary open angle glaucoma and exposed to the change of hydraulic pressure in a tissue culture flask connected to a glass syringe. The pressure was exerted by automatic infusion of the piston of the syringe and monitored by a pressure gauge. The intracellular calcium concentration was measured in real time with a calcium binding fluorescent dye, fluo-3. RESULTS--A small number (about 10%) of cells appearing morphologically to be trabecular cells showed transient elevations or oscillations of the intracellular calcium concentration in response to the elevation of hydraulic pressure to 20-30 mm Hg, indicating that a part of the human trabecular cells could sense the change in hydraulic pressure. CONCLUSION--Some cells in the human trabecular tissue seem to sense the change in intraocular pressure and might play a role in its regulation.
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机译：目的-为了了解眼压调节的机制，检查了人眼小梁细胞以确定它们是否可以响应液压的变化。方法-从小梁切除术中切除的小梁组织片段中培养的人小梁细胞，在三名原发性开角型青光眼患者的四只眼中进行暴露，并在与玻璃注射器相连的组织培养瓶中暴露于液压的变化。通过自动注入注射器的活塞施加压力，并通过压力计进行监控。用钙结合荧光染料fluo-3实时测量细胞内钙浓度。结果-少数（约10％）形态上看起来为小梁细胞的细胞显示出短暂的升高或细胞内钙浓度的振荡，这是由于液压升高至20-30 mm Hg而引起的，表明其中一部分人小梁细胞可以感知液压的变化。结论：人眼小梁组织中的某些细胞似乎感觉到眼压的变化，并可能在其调节中起作用。
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