1. NG-nitro-L-arginine (L-NOARG, 10(-4) M), an inhibitor of nitric oxide (NO) synthesis, had no contractile effect on isolated preparations of rabbit and human corpus cavernosum at baseline tension, but increased tension in preparations contracted by noradrenaline (rabbit 10(-5) M, man 3 x 10(-7)-3 x 10(-6) M) or K+ (rabbit 60 mM). 2. Electrical field stimulation (supramaximal voltage, 0.8 ms pulses, 5 s train duration, 0.5-35 Hz) of rabbit and human corpus cavernosum preparations contracted by noradrenaline (rabbit 10(-5) M, man 3 x 10(-6) M) or endothelin-1 (rabbit 10(-8) M) produced relaxations that were sensitive to tetrodotoxin (10(-6) M), and dependent on the frequency and number of pulses delivered. L-NOARG (10(-6)-10(-4) M), but not NG-nitro-D-arginine (D-NOARG, 10(-6)-10(-4) M), inhibited electrically induced relaxations in a concentration-dependent manner, and at 10(-4) M the relaxations were virtually abolished. L-Arginine (10(-3) M), but not D-arginine (10(-3) M), partly reversed the inhibitory effect of L-NOARG (10(-4) M). In rabbit corpus cavernosum preparations, as with Methylene Blue (3 x 10(-5) M), an inhibitor of the soluble guanylate cyclase, and haemoglobin (10(-5) M), sequestering NO in the extracellular space, significantly reduced electrically evoked relaxations. Scopolamine (10(-6) M) had little or no effect on relaxations induced by electrical field stimulation. 3. Preparations of rabbit and human corpus cavernosum contracted by noradrenaline (rabbit 10(-5) M, man 3 x 10(-6) M) were relaxed by carbachol (10(-9)-10(-4) M) in a concentration-dependent manner. Scopolamine (10(-6) M) and L-NOARG (10(-4) M) abolished, and Methylene Blue (3 x 10(-5) M) and haemoglobin (10(-5) M) greatly reduced, the carbachol-induced relaxation, while D-NOARG (10(-4) M) had no significant effect. 4. In rabbit corpus cavernosum preparations contracted by noradrenaline (10(-5) M), L-NOARG (10(-4) M) had no significant effect on relaxations induced by vasoactive intestinal polypeptide (10(-6) M). 5. SIN-1 (3-morpholino-sydnonimin hydrochloride, 10(-8)-3 x 10(-4) M), which spontaneously liberates NO, relaxed preparations of rabbit and human corpus cavernosum contracted by noradrenaline (rabbit 10(-5) M, man 3 x 10(-6) M) or endothelin-1 (rabbit 10(-8) M, man 3 x 10(-9) M) in a concentration-dependent way.(ABSTRACT TRUNCATED AT 400 WORDS)
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机译：1. NG-硝基-L-精氨酸（L-NOARG，10（-4）M）是一氧化氮（NO）合成的抑制剂，在基线张力下对兔和人海绵体的分离制剂没有收缩作用，但在去甲肾上腺素（兔子10（-5）M，男人3 x 10（-7）-3 x 10（-6）M）或K +（兔子60 mM）收缩的制剂中增加张力。 2.用去甲肾上腺素（兔10（-5）M，人3 x 10（-6））收缩的兔和人海绵体制剂的电场刺激（最大电压，0.8 ms脉冲，5 s训练持续时间，0.5-35 Hz）。 M）或内皮素1（兔10（-8）M）产生的松弛对河豚毒素（10（-6）M）敏感，并取决于所传递的脉冲的频率和数量。 L-NOARG（10（-6）-10（-4）M），而不是NG-硝基-D-精氨酸（D-NOARG，10（-6）-10（-4）M）抑制了电感应弛豫以浓度依赖的方式，在10（-4）M时，松弛几乎被消除。 L-精氨酸（10（-3）M），而不是D-精氨酸（10（-3）M），部分逆转了L-NOARG（10（-4）M）的抑制作用。在兔海绵体制剂中，与可溶性鸟苷酸环化酶抑制剂亚甲基蓝（3 x 10（-5）M）和血红蛋白（10（-5）M）隔离在细胞外空间中的NO明显减少了电引起放松。 Scopolamine（10（-6）M）对电场刺激引起的弛豫几乎没有影响。 3.用去甲肾上腺素（兔子10（-5）M，人3 x 10（-6）M）收缩的兔子和人海绵体的制备用卡巴胆碱（10（-9）-10（-4）M）放松。浓度依赖性的方式。废除co草胺（10（-6）M）和L-NOARG（10（-4）M），亚甲基蓝（3 x 10（-5）M）和血红蛋白（10（-5）M）大大减少，卡巴胆碱引起的放松，而D-NOARG（10（-4）M）没有明显的作用。 4.在去甲肾上腺素（10（-5）M）收缩的兔海绵体制剂中，L-NOARG（10（-4）M）对血管活性肠多肽（10（-6）M）诱导的松弛没有明显影响。 5. SIN-1（3-吗啉代-亚砜胺盐酸盐，10（-8）-3 x 10（-4）M），可自发释放NO，并通过去甲肾上腺素收缩使兔和人海绵体（兔子10（- 5）M，man 3 x 10（-6）M）或内皮素-1（兔子10（-8）M，man 3 x 10（-9）M）（摘要截短为400字） ）
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