The acute effects of strength endurance and concurrentexercises on the Akt/mTOR/p70S6K1 and AMPK signaling pathway responses inrat skeletal muscle

摘要

The activation of competing intracellular pathways has been proposed to explain the reduced training adaptations after concurrent strength and endurance exercises (CE). The present study investigated the acute effects of CE, strength exercises (SE), and endurance exercises (EE) on phosphorylated/total ratios of selected AMPK and Akt/mTOR/p70^S6K1 pathway proteins in rats. Six animals per exercise group were killed immediately (0 h) and 2 h after each exercise mode. In addition, 6 animals in a non-exercised condition (NE) were killed on the same day and under the same conditions. The levels of AMPK, phospho-Thr¹⁷²AMPK (p-AMPK), Akt, phospho-Ser⁴⁷³Akt (p-Akt), p70^S6K1, phospho-Thr³⁸⁹-p70^S6K1 (p-p70^S6K1), mTOR, phospho-Ser²⁴⁴⁸mTOR (p-mTOR), and phospho-Thr¹⁴⁶²-TSC2 (p-TSC2) expression were evaluated by immunoblotting in total plantaris muscle extracts. The only significant difference detected was an increase (i.e., 87%) in Akt phosphorylated/total ratio in the CE group 2 h after exercise compared to the NE group (P = 0.002). There were no changes in AMPK, TSC2, mTOR, or p70^S6K1 ratios when the exercise modes were compared to the NE condition (P ≥ 0.05). In conclusion, our data suggest that low-intensity and low-volume CE might not blunt thetraining-induced adaptations, since it did not activate competing intracellularpathways in an acute bout of strength and endurance exercises in rat skeletalmuscle.