1. A method for detecting the activation of individual myofibrils or groups of myofibrils within an isolated muscle fibre is described. It consists in making all the myofibrils wavy by setting the fibre in gelatine and compressing it longitudinally; active shortening of myofibrils can then be recognized by the straightening out of the waves.2. The time course of this straightening during a twitch was found by high-speed ciné micrography.3. There is a delay of activation between the superficial and central myofibrils, from which the velocity of inward spread of activation can be found.4. This velocity has a Q10 of 2, and is about 7 cm/sec at 20° C. The mechanism of the inward spread of activation is discussed.5. On relaxation the waves reappear, showing that there is a spontaneous elongation of the myofibrils.
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机译:1.描述了一种用于检测分离的肌纤维内单个肌原纤维或肌原纤维组的活化的方法。通过将纤维置于明胶中并纵向压缩,使所有肌原纤维呈波浪形。通过理顺波浪,可以识别肌原纤维的有效缩短。2。高速ciné显微术发现了抽搐期间这种矫直的时间过程。3。表层和中央肌原纤维之间存在激活延迟,从中可以发现激活向内扩散的速度。4。该速度的Q10为2,在20°C下约为7 cm / sec。讨论了激活向内扩展的机制。5。放松后,波重新出现,表明肌原纤维自发地伸长。
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