Onset of contractility in cardiac muscle

摘要

1. A technique is described whereby (i) quick stretches and releases of controlled velocity, amplitude and time of onset can be applied to muscle. (ii) Releases from isometric to isotonic contraction can be performed at controlled delays relative to the stimulus, and displayed on a delayed expanded oscilloscope sweep. An isotonic lever system with an equivalent mass of 12·8 mg is described.2. Quick stretch of rabbit or cat papillary muscle after excitation does not result in a level of tension equal to or greater than normal peak isometric tension appropriate to the stretched length. Stretches applied during the first half of the rising phase of tension development give responses nearly identical to the same stretches applied before the stimulus (indicating that Starling's Law of the heart holds until this time). Stretches applied in the later phase of tension development or during relaxation result in diminished peak isometric tensions or accelerated relaxation.3. The rate of tension development following quick releases of isometrically contracting muscle to zero tension is not maximal until the releases are made 150-200 msec after excitation.4. Shortening velocity with light afterloads is not initially maximal nor constant for an appreciable period of time. The shortening velocity with heavy afterloads reaches its maximum more rapidly when the load is not lifted within the first 200 msec of a contraction which, if maintained isometric, would have required 400-500 msec to reach peak tension. With these heavier loads, a period of 100-200 msec of constant shortening velocity may occur.5. Freeloaded isotonic contractions show an inflexion in their shortening curves occurring 150-200 msec after excitation.6. Maximum rate of isotonic shortening following releases from isometric to isotonic contraction with a given load is not maximal until the releases occur about 200 msec after the stimulus.7. It is concluded that contractility in cardiac muscle is relatively slow in its onset with maximum capacity to shorten occurring about midway through the rising phase of isometric tension development.