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Data on the enzymatic conversion of alkaline peroxide oxidative pretreated sugarcane bagasse for the production of fermentable sugars

机译:碱性过氧化物氧化预处理甘蔗渣生产可发酵糖的酶促转化数据

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摘要

Central composite design (CCD) approach of the response surface methodology design of experiment was adopted to determine the production of fermentable sugars after enzymatic conversion of alkaline peroxide oxidative pretreated sugarcane bagasse lignocellulose. MINITAB 16 statistical software was used to design the experiments, evaluate and interpret data generated during the process. The effects of factors such as time, hydrogen peroxide concentration, and temperature on treated biomass for reducing sugars (RS) production were investigated. Operating pretreatment conditions (low–high design levels) were reaction time (6–10 h), hydrogen peroxide concentrations (1–3%v/v), and reaction temperature (60–90 °C). With the desirability of optimization of 1.000, optimal reducing sugar yield after enzymatic hydrolysis was validated to be at 100.2 °C, reaction time of 4.6 h, and hydrogen peroxide concentration of 0.3% with optimum RS yield of 153.74 mg equivalent glucose/g biomass.
机译:采用响应面法的中央复合设计法(CCD),通过碱性过氧化物氧化预处理的甘蔗渣木质纤维素的酶转化,确定发酵糖的产量。 MINITAB 16统计软件用于设计实验,评估和解释过程中生成的数据。研究了时间,过氧化氢浓度和温度等因素对经处理的生物质的还原糖(RS)产生的影响。操作预处理条件(低至高设计水平)为反应时间(6-10小时),过氧化氢浓度(1-3%v / v)和反应温度(60-90°C)。理想的优化为1.000,酶水解后的最佳还原糖产量经验证为100.2°C,反应时间4.6 h,过氧化氢浓度为0.3%,最佳RS产量为153.74 mg当量葡萄糖/ g生物质。

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