Effect of cyclin G2 on proliferative ability of SGC-7901 cell

摘要

AIM: To study the effect of cyclin G2 on proliferation of gastric adenocarcinoma cell line-SGC-7901 cell in vitro.METHODS: By use of cation lipofectamine transfection reagent, the pIRES-G2 and pIRESneo plasmids were transferred into SGC-7901cell line. Anticlones were selected by G418. Positive clones were observed and counted using Giemsa staining. Cell proliferative ability was assayed by MTT.RESULTS: (1) The clone number of pIRES-G2 group decreased, clone volume reduced. The number of cell clones in pIRESneo group was 87 ± 3, that of pIRES-G2 group was 53 ± 4, occupying 60.1% of pIRESneo group, there was significant difference obviously (P < 0.01, t = 15.45). (2) The average absorbance of clone cell obtained by stable transfection of pIRES-G2 at 570 nm was 1.6966 ± 0.2125, the average absorbance of clone cell obtained by stable transfection of pIRESneo at 570 nm was 2.1182 ± 0.3675, there was significant difference between them (P < 0.01, t = 3.412).CONCLUSION: Cyclin G2 can inhibit SGC-7901cell proliferative ability obviously, it may be a negative regulator in cell cycle regulation.