Effect of Chelidonine on the expressions of Cdk1 and cyclinB1 proteins in SGC-7901 cells

摘要

To study the effect of chelidonine on Cdk1, p-Cdk1( Thr14), cyclinB1 protein expression in SGC-7901 Cells, and to explore the mechanism of chelidonine inducing G2/M phase arrest in SGC-7901 Cells. Western Blotting was used to study the effects of chelidonine on the Cdk1, p-Cdk1(Thr14) and cyclinB1 protein expressions in SGC-7901 cells. Chelidonine could significantly decrease the expression of Cdk1 and cyclinB1 protein. Meanwhile, chelidonine could remarkably increase the expression of p-Cdk1(Thr14) protein in a dose dependent manner. Chelidonine can down-regulate the expression of Cdk1, cyclinB1 and up- regulate p-Cdk1 (Thr14) protein expression in SGC-7901 cells，which may be one of the role mechanism of G_2 /M phase arrest induced by chelidonine in SGC-7901 cells which may be one of the role mechanism of G_2 /M phase arrest induced by chelidonine in SGC-7901 Cells.