The workflow begins with the collection of coastal marine waters for downstream microbial community, nutrient and trace gas analyses. For this method, samples were collected from the deck of the HMS John Strickland operating in Saanich Inlet. This video documents large volume (≥20 L) filtration of microbial biomass, ranging between 0.22μm and 2.7μm in diameter, from the water column. Two 20L samples can be filtered simultaneously using a single pump unit equipped with four rotating heads. Filtration is done in the field on extended trips, or immediately upon return for day trips. It is important to record the amount of water passing through each sterivex filter unit. To prevent biofilm formation between sampling trips, all filtration equipment must be rinsed with dilute HCl and deionized water and autoclaved immediately after use. This procedure will take approximately 5 hours plus an additional hour for clean up.
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机译：该工作流程始于收集沿海海水以用于下游微生物群落，营养物和微量气体分析。对于此方法，从在萨尼奇（Sanich）入口运营的HMS John Strickland的甲板上收集样品。该视频记录了从水柱中对微生物生物质进行的大体积（≥20 L）过滤，直径范围为0.22μm至2.7μm。使用配有四个旋转头的单个泵单元可以同时过滤两个20升样品。长时间旅行或在返回一日游后立即在现场进行过滤。记录通过每个无菌过滤器单元的水量很重要。为了防止两次取样之间形成生物膜，所有过滤设备必须用稀HCl和去离子水冲洗，并在使用后立即进行高压灭菌。此过程大约需要5个小时，加上一个小时的清理时间。
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