The influence of cell concentration at cryopreservation on neutrophil engraftment after autologous peripheral blood stem cell transplantation

摘要

BackgroundPeripheral blood stem cell concentrations are traditionally adjusted to 20–40 × 10⁶ leukocytes/mL prior to freezing. This low cell concentration at cryopreservation implies larger volumes with more dimethyl sulfoxide being used, and higher cost and toxicity at the time of transplant. Higher cell concentrations have been reported but this is not widely accepted. Moreover, the influence of cell concentration on engraftment has not been well documented. Therefore, this study retrospectively analyzed the influence of peripheral blood stem cell concentration at freezing on engraftment after autologous hematopoietic stem cell transplantation.