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Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.)

机译:鉴定藏红花(Crocus sativus L.)中与胚发生性和非胚发生性愈伤组织相关的差异积累蛋白

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摘要

BackgroundSomatic embryogenesis (SE) is a complex biological process that occurs under inductive conditions and causes fully differentiated cells to be reprogrammed to an embryo like state. In order to get a better insight about molecular basis of the SE in Crocus sativus L. and to characterize differentially accumulated proteins during the process, a proteomic study based on two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry has been carried out.
机译:背景技术体细胞胚胎发生(SE)是一个复杂的生物过程,在诱导条件下发生,并导致完全分化的细胞重新编程为类似胚胎的状态。为了更好地了解番红花中SE的分子基础并鉴定过程中差异积累的蛋白质,基于二维凝胶电泳和基质辅助激光解吸/电离飞行时间的蛋白质组学研究光谱法已经进行。

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