首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Conserved in situ arrangement of complex I and III2 in mitochondrial respiratory chain supercomplexes of mammals yeast and plants
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Conserved in situ arrangement of complex I and III2 in mitochondrial respiratory chain supercomplexes of mammals yeast and plants

机译:哺乳动物酵母和植物的线粒体呼吸链超复合物中复合物I和III2的保守原位排列

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摘要

We used electron cryo-tomography and subtomogram averaging to investigate the structure of complex I and its supramolecular assemblies in the inner mitochondrial membrane of mammals, fungi, and plants. Tomographic volumes containing complex I were averaged at ∼4 nm resolution. Principal component analysis indicated that ∼60% of complex I formed a supercomplex with dimeric complex III, while ∼40% were not associated with other respiratory chain complexes. The mutual arrangement of complex I and III2 was essentially conserved in all supercomplexes investigated. In addition, up to two copies of monomeric complex IV were associated with the complex I1III2 assembly in bovine heart and the yeast Yarrowia lipolytica, but their positions varied. No complex IV was detected in the respiratory supercomplex of the plant Asparagus officinalis. Instead, an ∼4.5-nm globular protein density was observed on the matrix side of the complex I membrane arm, which we assign to γ-carbonic anhydrase. Our results demonstrate that respiratory chain supercomplexes in situ have a conserved core of complex I and III2, but otherwise their stoichiometry and structure varies. The conserved features of supercomplex assemblies indicate an important role in respiratory electron transfer.
机译:我们使用电子冷冻断层摄影术和亚断层摄影术平均来研究哺乳动物,真菌和植物的线粒体内膜中复合物I及其超分子组装体的结构。包含复合物I的层析成像体积的平均分辨率约为4 nm。主成分分析表明,约60%的复合物I与二聚体复合物III形成超复合物,而约40%的复合物与其他呼吸链复合物无关。在所有研究的超复合物中,复合物I和III2的相互排列基本上是保守的。另外,在牛心脏和解脂耶氏酵母中,多达两个拷贝的单体复合物IV与复合物I1III2装配相关,但它们的位置各不相同。在植物芦笋的呼吸超复合物中未检测到复合物IV。相反,在复合体I膜臂的基质侧观察到了约4.5 nm的球状蛋白密度,我们将其分配为γ-碳酸酐酶。我们的结果表明,原位呼吸链超复合体具有复合体I和III2的保守核,但其化学计量和结构不同。超复杂组件的保守特征表明在呼吸电子转移中起重要作用。

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