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Molecular model of a type III secretion system needle: Implications for host-cell sensing

机译:III型分泌系统针的分子模型:对宿主细胞感测的意义

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摘要

Type III secretion systems are essential virulence determinants for many Gram-negative bacterial pathogens. The type III secretion system consists of cytoplasmic, transmembrane, and extracellular domains. The extracellular domain is a hollow needle protruding above the bacterial surface and is held within a basal body that traverses both bacterial membranes. Effector proteins are translocated, via this external needle, directly into host cells, where they subvert normal cell functions to aid infection. Physical contact with host cells initiates secretion and leads to formation of a pore, thought to be contiguous with the needle channel, in the host-cell membrane. Here, we report the crystal structure of the Shigella flexneri needle subunit MxiH and a complete model for the needle assembly built into our three-dimensional EM reconstruction. The model, combined with mutagenesis data, reveals that signaling of host-cell contact is relayed through the needle via intersubunit contacts and suggests a mode of binding for a tip complex.
机译:III型分泌系统是许多革兰氏阴性细菌病原体必不可少的毒力决定因素。 III型分泌系统由细胞质,跨膜和细胞外结构域组成。细胞外结构域是突出于细菌表面上方的空心针,并被固定在穿过两个细菌膜的基体内。效应蛋白通过该外部针头直接转移到宿主细胞中,在那里它们破坏正常的细胞功能以帮助感染。与宿主细胞的物理接触会启动分泌,并导致在宿主细胞膜中形成一个孔,该孔被认为与针通道相邻。在这里,我们报告了志贺氏志贺氏菌针头亚基MxiH的晶体结构,以及在我们的三维EM重建中内置的针头组件的完整模型。该模型与诱变数据相结合,揭示宿主细胞接触的信号是通过亚单位间接触通过针传递的,并暗示了尖端复合物的结合方式。

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