α-Amylase Synthesis in Wheat Kernels as Influenced by the Seed Coat

摘要

The effect of seed coat removal on the synthesis of α-amylase isoenzymes in wheat was investigated. The immature wheat endosperm-aleurone (seed coat and embryo detached) produced considerably less α-amylase activity than immature whole or de-embryonated wheat kernels, when incubated under identical conditions of 18.5 C and 99% humidity, in the presence or absence of gibberellic acid (GA3). The incubated endosperm-aleurone also exhibited unique α-amylase isoenzyme composition when compared to the isoenzyme compositions of incubated whole and de-embryonated immature and mature wheat kernels both in the presence or absence of GA3. Subsequent studies indicated that the seed coat may contain factor(s) required for normal α-amylase isoenzyme synthesis.