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Correlation between observed transcripts and sequenced ORFs of chromosome III of Saccharomyces cerevisiae.

机译:酿酒酵母Ⅲ号染色体的观察到的转录本与测序的ORF之间的相关性。

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摘要

Most of the 155 transcripts of the genes on chromosome III of S.cerevisiae that were identified by a series of Northern hybridization experiments (Yoshikawa, A. and Isono, K., Yeast 6, 383-401, 1990) have been correlated with the open reading frames (ORFs) deduced from the nucleotide sequence data for this chromosome (Oliver, S.G. et al., Nature 357, 38-46, 1992). This was performed by comparing the experimentally constructed physical map and the one produced from the nucleotide sequence data, and then the sizes and positions of observed transcripts and those of sequenced ORFs. The data thus provide information for possible products of the sequenced ORFs (genes) of chromosome III of S.cerevisiae as well as their likely levels of expression in vegetatively growing cells.
机译:通过一系列Northern杂交实验(Yoshikawa,A.和Isono,K.,Yeast 6,383-401,1990)鉴定的酿酒酵母III染色体上的155个转录本中的大多数已经与由该染色体的核苷酸序列数据推导的开放阅读框(ORF)(Oliver,SG等人,Nature 357,38-46,1992)。通过比较实验构建的物理图谱和从核苷酸序列数据生成的图谱,然后比较观察到的转录本和测序的ORF的大小和位置来执行此操作。因此,数据提供了酿酒酵母III号染色体测序ORF(基因)可能产物的信息,以及它们在营养生长细胞中可能的表达水平。

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