Dihydrochalcones and Diterpenoids from Pteris ensiformis and Their Bioactivities

摘要

Two new dihydrochalcone enantiomers (+)->1 and (−)->1, along with eight known compounds >3–>10, were obtained from Pteris ensiformis. The planar structures were determined on the basis of extensive 1D and 2DNMR and HRESIMS. The resolution of (+)->1 and (−)->1 was achieved by chiral HPLC analysis. The absolute configurations of (+)->1 and (−)->1 were established by the bulkiness rule using Rh2(O2CCF3)4-induced circular dichroism (ICD) method. Compounds (+)->1, (−)->1, >8, >9 and >10 exhibited the inhibitory assay of NO production in mouse macrophages stimulated by LPS, with IC50 values of 2.0, 2.5, 8.0, 9.5 and 5.6 μM, respectively. Otherwise, compound >10 showed moderate cytotoxic activity against HCT-116, HepG-2 and BGC-823 cell lines with IC50 values of 3.0, 10.5 and 6.3 μM, respectively.