Acorns play an important role in human history and are a source of food and recipes for many cultures around the world. In this study, eleven oleanolic triterpenes, one of which was novel, were isolated from Chinese acorns (Quercus serrata var. brevipetiolata). The chemical structure of the novel triterpene, which was identified as 2α,3β,19α-trihydroxy-24-oxo-olean-12-en-28-oic acid (>1), was established based on the interpretation of chemical and spectroscopic analyses, including IR, HR-ESI-MS, and NMR experiments (1H, 13C NMR, DEPT, 1H-1H COSY, HSQC, HMBC, and NOESY). All isolated compounds were tested for their inhibitory effects on LPS-induced nitric oxide (NO) production in RAW 264.7 macrophages. Compared with the positive control drug indomethacin (IC50 = 47.4 μM), compounds >1, >3, >6 and >8 exhibited remarkable anti-inflammatory activities with IC50 values of 5.4, 7.8, 4.0 and 8.9 μM, respectively. Besides, compounds >2, >4, >7 and >9 also showed moderate anti-inflammatory activities with IC50 values of 10.1, 13.0, 20.1 and 17.2 μM, respectively. Furthermore, Compound >1 could inhibit TNF-α-induced IL-6 and IL-8 production in MH7A cells.
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机译:叶绿素/壳聚糖/小球藻/铁/钙/ DHA / EPA /含绿豆/黑豆/红豆/白豆室温暴露,绿豆芽/绿色寄主豆芽/大白菜/新鲜/羽衣甘蓝/马铃薯/大蒜/姜/生姜/黄瓜/南瓜/菠菜/人参/红鸭/迷你番茄/西瓜/瓜,嫩芽/马铃薯/甘薯/黄瓜/西瓜/瓜/大白菜/大蒜/生姜/普通大米,阿克巴里,大米/大米/大麦/荞麦/橡子塑料空气分配孔,把手,阳光遮蔽的盖子,水分配孔处理幼苗,处理水耕栽培容器,不良,岩石形成,固体培养基,水耕,稻田,田地,耕种