Serum organochlorine pesticides residues and risk of cancer: A case-control study

摘要

Organochlorine pesticides (OCPs) are frequently used worldwide as insecticides, fungicides, herbicides and termiticides and have been associated with a variety of cancers in animal and human studies. In the present study, we examined residues of fourteen OCPs in the serum samples of diagnosed cancer patients and healthy residents of Karachi, Pakistan. A random collection of fasting blood samples was carried out from the donors with informed consent. Serum was separated within 2 h of blood collection and was then subjected to extraction with organic solvents followed by purification with florisil column. The final organic extract of each serum sample was processed with Gas Chromatograph coupled with Electron Capture Detector (GC-ECD). OCPs were detected in 97.59% of the cancer cases and 93.75% of the healthy subjects. Mean concentrations of total OCPs (ΣOCPs) was found elevated in the cancer group (0.606 mg/kg) compared with the control group (0.322 mg/kg). Endosulfan was the highest prevalent OCP with a mean concentration of 0.214 mg/kg in the cancer group and 0.166 mg/kg in the control group. The second most prevalent OCP was 4,4-DDE with a mean concentration of 0.131 mg/kg in the cancer group and 0.019 mg/kg in the control group. Highest level of ΣOCPs was detected in the breast cancer cases (20.411 mg/kg) with a mean level of (2.041 mg/kg). In light of the obtained results and available literature on the subject, it has been concluded that OCPs are positively associated with the risk of various cancers in humans.