Previous studies have demonstrated that Shadoo (Sho), a GPI-linked glycoprotein encoded by the Sprn gene with a membrane localization similar to PrPC, is reduced in the brains of rodents with terminal prion disease. To determine the functional significance of Sho in prion disease pathogenesis, Sho-deficient mice were generated by gene targeting. Sho knockout and control wild-type (WT) mice were infected with themouse-adapted scrapie strains 22L or RML. No significant differences in survival, the incubation period of prion disease or other disease features were observed between Sho mutant and WT mice. In this model of prion disease, Sho removal had no effect on disease pathogenesis.
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机译：先前的研究表明，由Sprn基因编码的GPI连接的糖蛋白Shadoo（Sho）具有类似于PrP C 的膜定位，在患有terminal病毒的啮齿类动物的大脑中其含量降低了。为了确定Sho在病毒疾病发病机理中的功能意义，通过基因靶向产生了Sho缺陷小鼠。将Sho敲除和对照野生型（WT）小鼠用适应小鼠的瘙痒病菌株22L或RML感染。在Sho突变体和WT小鼠之间未观察到存活率，of病毒疾病的潜伏期或其他疾病特征的显着差异。在这种of病毒疾病模型中，去除Sho对疾病的发病机理没有影响。
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