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Discrimination between endemic and feedborne Salmonella Infantis infection in cattle by molecular typing.

机译:通过分子分型区分牛的地方性和饲料源性沙门氏菌沙门氏菌感染。

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摘要

Salmonella enterica serovar Infantis is endemic in Finnish cattle. Feed contaminated with S. Infantis was distributed to cattle farms in May 1995. Following increased sampling, S. Infantis was detected on 242 farms in 1995. Molecular typing was used to differentiate the farms that were infected by the feed-related Infantis from those infected by other endemic strains. Twenty-three isolates from feed in 1995 and 413 from cattle (72 from 19924, 324 from 1995, 17 from 1996-7) were analysed. The feed-related Infantis was clonally related to the endemic infection by the ribotype, IS200-type and XbaI-profile. The feed isolates had a distinctive plasmid that appeared in pulsed-field gel electrophoresis as a 60 kb band when cleaved with XbaI or linearized by S1-nuclease. This plasmid appeared in cattle only since the outbreak and seemed stable on the follow-up farms. In addition to contact farms, the feedborne strain was found on 19% of the farms infected with S. Infantis in 1995 but not having bought suspected feedstuffs, possibly as secondary infections.
机译:沙门氏菌肠型沙门氏菌是芬兰牛的地方病。 1995年5月,被S. Infantis污染的饲料分发给了养牛场。随着采样的增加,1995年在242个农场中检测到了S. Infantis。使用分子分型将与饲料相关的Infantis感染的农场与感染的农场区分开来。通过其他地方性毒株。分析了1995年饲料中的23株分离株和牛中的413株(19924年的72株,1995年的324株,1996-7年的17株)。饲料相关的婴幼儿通过核糖型,IS200型和XbaI谱与地方性感染呈克隆关系。饲料分离物具有独特的质粒,当用XbaI裂解或通过S1-核酸酶线性化时,在脉冲场凝胶电泳中显示为60 kb的条带。该质粒仅在疫情爆发后才出现在牛中,并且在后续农场中似乎稳定。除接触式养殖场外,1995年在感染沙门氏菌的19%的养殖场中发现了食源性菌株,但未购买可疑饲料,可能是继发感染。

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