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RapaNet: A Web Tool for the Co-Expression Analysis of Brassica rapa Genes

机译:RapaNet:用于芸苔属rapa基因共表达分析的Web工具

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摘要

Accumulated microarray data are used for assessing gene function by providing statistical values for co-expressed genes; however, only a limited number of Web tools are available for analyzing the co-expression of genes of Brassica rapa. We have developed a Web tool called RapaNet (), which is based on a data set of 143 B rapa microarrays compiled from various organs and at different developmental stages during exposure to biotic or abiotic stress. RapaNet visualizes correlated gene expression information via correlational networks and phylogenetic trees using Pearson correlation coefficient (r). In addition, RapaNet provides hierarchical clustering diagrams, scatterplots of log ratio intensities, related pathway maps, and cis-element lists of promoter regions. To ascertain the functionality of RapaNet, the correlated genes encoding ribosomal protein (L7Ae), photosystem II protein D1 (psbA), and cytochrome P450 monooxygenase in glucosinolate biosynthesis (CYP79F1) were retrieved from RapaNet and compared with their Arabidopsis homologues. An analysis of the co-expressed genes revealed their shared and unique features.
机译:通过提供共表达基因的统计值,将累积的微阵列数据用于评估基因功能;但是,仅有限数量的Web工具可用于分析甘蓝型油菜基因的共表达。我们已经开发了一种称为RapaNet()的Web工具,该工具基于143 B rapa微阵列的数据集,该数据是从暴露于生物或非生物胁迫期间各个器官和处于不同发育阶段的数据汇编而来的。 RapaNet使用Pearson相关系数(r)通过相关网络和系统树将相关的基因表达信息可视化。此外,RapaNet还提供了层次化的聚类图,对数比强度的散点图,相关的路径图以及启动子区域的顺式元素列表。为了确定RapaNet的功能,从RapaNet检索了芥子油苷生物合成(CYP79F1)中编码核糖体蛋白(L7Ae),光系统II蛋白D1(psbA)和细胞色素P450单加氧酶的相关基因,并将其与拟南芥的同系物进行了比较。对共表达基因的分析揭示了它们共有和独特的特征。

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