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首页> 美国卫生研究院文献>G3: GenesGenomesGenetics >Vps74 Connects the Golgi Apparatus and Telomeres in Saccharomyces cerevisiae
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Vps74 Connects the Golgi Apparatus and Telomeres in Saccharomyces cerevisiae

机译：Vps74连接酿酒酵母中的高尔基体和端粒

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In mammalian cell culture, the Golgi apparatus fragment upon DNA damage. GOLPH3, a Golgi component, is a phosphorylation target of DNA-PK after DNA damage and contributes to Golgi fragmentation. The function of the yeast (Saccharomyces cerevisiae) ortholog of GOLPH3, , in the DNA damage response has been little studied, although genome-wide screens suggested a role at telomeres. In this study we investigated the role of at telomeres and in the DNA damage response. We show that decreases the fitness of telomere defective -1 cells and contributes to the fitness of Δ cells. Importantly, loss of in Δ cells exacerbates the temperature dependent growth defects of these cells in a and -dependent manner. Furthermore, reduces the fitness of Δ Δ cells suggesting that ssDNA contributes to the fitness defects of Δ Δ cells. Systematic genetic interaction analysis of Δ, Δ and href="http://www.yeastgenome.org/locus/S000004897/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">yku70Δ href="http://www.yeastgenome.org/locus/S000002780/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">vps74Δ cells suggests that href="http://www.yeastgenome.org/locus/S000002780/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">vps74Δ causes a milder but similar defect to that seen in href="http://www.yeastgenome.org/locus/S000004897/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">yku70Δ cells. href="http://www.yeastgenome.org/locus/S000002780/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">vps74Δ cells have slightly shorter telomeres and loss of href="http://www.yeastgenome.org/locus/S000002780/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">VPS74 in href="http://www.yeastgenome.org/locus/S000004897/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">yku70Δ or href="http://www.yeastgenome.org/locus/S000004837/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">mre11Δ cells further shortens the telomeres of these cells. Interestingly, loss of href="http://www.yeastgenome.org/locus/S000002780/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Vps74 leads to increased levels of href="http://www.yeastgenome.org/locus/S000002489/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Stn1, a partner of href="http://www.yeastgenome.org/locus/S000002379/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Cdc13 in the CST telomere capping complex. Overexpression of href="http://www.yeastgenome.org/locus/S000002489/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Stn1 was previously shown to cause telomere shortening, suppression of href="http://www.yeastgenome.org/locus/S000002379/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">cdc13-1 and enhancement of href="http://www.yeastgenome.org/locus/S000004897/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">yku70Δ growth defects, suggesting that increased levels of href="http://www.yeastgenome.org/locus/S000002489/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Stn1 may be the route by which href="http://www.yeastgenome.org/locus/S000002780/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Vps74 affects telomere function. These results establish href="http://www.yeastgenome.org/locus/S000002780/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5940170&issue-id=312340&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Vps74 as a novel regulator of telomere biology.
机译：在哺乳动物细胞培养中，高尔基体在DNA受损时会断裂。 GOLPH3是高尔基体的一种成分，是DNA损伤后DNA-PK的磷酸化靶标，并有助于高尔基体片段化。尽管全基因组筛选提示端粒起作用，但对GOLPH3的酵母（Saccharomyces cerevisiae）直系同源物在DNA损伤反应中的功能研究很少。在这项研究中，我们研究了端粒和DNA损伤反应中的作用。我们表明，降低端粒缺陷-1细胞的适应性并有助于Δ细胞的适应性。重要的是，Δ细胞的损失以α依赖性方式加剧了这些细胞的温度依赖性生长缺陷。此外，降低了ΔΔ细胞的适应性，表明ssDNA导致了ΔΔ细胞的适应性缺陷。 Δ，Δ和href =“ http://www.yeastgenome.org/locus/S000004897/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-的系统遗传相互作用分析id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> yku70 Δhref =” http://www.yeastgenome.org/locus/S000002780 / overview“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> vps74 Δ单元格表明href =“ http://www.yeastgenome.org/locus/S000002780/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> vps74 Δ会导致较轻微但与href =” http：//相似的缺陷www.yeastgenome.org/locus/S000004897/overview“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆art icle-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> yku70 Δ细胞。 href =“ http://www.yeastgenome.org/locus/S000002780/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article ％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> vps74 Δ细胞的端粒略短，丢失了href =” http://www.yeastgenome.org/locus/S000002780/概述” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI” target =“ _ blank”> VPS74 < / a>在href =“ http://www.yeastgenome.org/locus/S000004897/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> yku70 Δ或href =” http://www.yeastgenome.org/locus/S000004837/overview“数据-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront ％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> mre11 Δ细胞进一步缩短了这些细胞的端粒。有趣的是，href =“ http://www.yeastgenome.org/locus/S000002780/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id的丢失= 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Vps74 导致href =” http://www.yeastgenome.org/locus/S000002489/概述” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI” target =“ _ blank”> Stn1 < / a>，href =“ http://www.yeastgenome.org/locus/S000002379/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = CST端粒上限复合物中的312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Cdc13 。 href =“ http://www.yeastgenome.org/locus/S000002489/overview”的过度表达data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Stn1 曾导致端粒缩短，禁止href =“ http://www.yeastgenome.org/locus/S000002379/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> cdc13 -1和 href =” http://www.yeastgenome.org/locus/ S000004897 /概述“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> yku70 Δ生长不良，表明href =“ http://www.yeastgenome.org/locus/S000002489/overview” data-ga-action =“ click_feat_suppl” ref = “ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI” target =“ _ blank”> Stn1 可能是href = “ http://www.yeastgenome.org/locus/S000002780/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆arti cle-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Vps74 影响端粒功能。这些结果建立了href =“ http://www.yeastgenome.org/locus/S000002780/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5940170＆issue-id = 312340＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Vps74 作为端粒生物学的新型调节剂。

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