Physical appearance traits, such as feather-crested head, comb size and type, beard, wattles size, and feathered feet, are used to distinguish between breeds of chicken and also may be associated with economic traits. In this study, a genome-wide linkage analysis was used to identify candidate regions and genes for physical appearance traits and to potentially provide further knowledge of the molecular mechanisms that underlie these traits. The linkage analysis was conducted with an F2 population derived from Beijing-You chickens and a commercial broiler line. Single-nucleotide polymorphisms were analyzed using the Illumina 60K Chicken SNP Beadchip. The data were used to map quantitative trait loci and genes for six physical appearance traits. A 10-cM/0.51-Mb region (0.0−10.0 cM/0.00−0.51 Mb) with 1% genome-wide significant level on LGE22C19W28_E50C23 linkage group (LGE22) for crest trait was identified, which is likely very closely linked to the HOXC8. A QTL with 5% chromosome-wide significant level for comb weight, which partly overlaps with a region identified in a previous study, was identified at 74 cM/25.55 Mb on chicken (Gallus gallus; GG) chromosome 3 (i.e., GGA3). For beard and wattles traits, an identical region 11 cM/2.23 Mb (0.0−11.0 cM/0.00−2.23 Mb) including WNT3 and GH genes on GGA27 was identified. Two QTL with 1% genome-wide significant level for feathered feet trait, one 9-cM/2.80-Mb (48.0-57.0/13.40-16.20 Mb) region on GGA13, and another 12-cM/1.45-Mb (41.0−53.0 cM/11.37−12.82 Mb) region on GGA15 were identified. These candidate regions and genes provide important genetic information for the physical appearance traits in chicken.
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机译:物理外观特征,例如羽毛冠状头,梳子的大小和类型,胡须,荆棘的大小和羽毛状的脚,被用来区分鸡肉的品种,也可能与经济性状有关。在这项研究中,全基因组连锁分析被用来识别物理外观特征的候选区域和基因,并可能提供有关这些特征基础的分子机制的进一步知识。连锁分析是利用北京幼鸡和商品肉鸡生产线的F2种群进行的。使用Illumina 60K Chicken SNP Beadchip分析单核苷酸多态性。数据用于绘制六个物理外观性状的数量性状基因座和基因。 LGE22C19W28_E50C23连锁特征的10-cM / 0.51-Mb区域(0.0-10.0 cM / 0.00-0.51 Mb)在基因组范围内具有1%的全基因组显着水平,这与波峰性状相关,这很可能与HOXC8紧密相关。鸡(鸡)(Gallus gallus; GG)3号染色体(即GGA3)的QTL在全染色体上具有5%的染色体显着权重,与先前研究中确定的区域部分重叠,被确定为74 cM / 25.55 Mb。对于胡须和胡桃性状,鉴定出包含GGA27上的WNT3和GH基因的11 cM / 2.23 Mb(0.0-11.0 cM / 0.00-2.23 Mb)相同区域。两个QTL具有1%的全基因组显着水平的羽毛足性状,一个GGA13上的9-cM / 2.80-Mb(48.0-57.0 / 13.40-16.20 Mb)区域,另一个12-cM / 1.45-Mb(41.0-53.0确定了GGA15上的cM / 11.37-12.82 Mb)区域。这些候选区域和基因为鸡的外观特征提供重要的遗传信息。
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