The Separation and Antigenic Characteristics of Some Fractions of Papain Digests of Human Serum Gamma Globulins and the Antigenic Relationships of Some Human Gamma Globulins with the Gamma Globulins of Some Other Mammalian Sera

摘要

1. Papain digests of human γ globulin (7S γ globulin) containing slow (S) and fast (F) immunoelectrophoretic components were fractionated by chromatography on diethylaminoethyl cellulose and by salting out with ammonium sulphate. No direct correlation was found between the antigenic characteristics and the chromatographic and electrophoretic properties of the S component fractionated by these methods.2. β2M globulin (γ1 macroglobulin or 19S γ globulin) contained the S component of γ globulin, but the F component was not detected.3. Rhesus monkey γ globulin more readily absorbed the anti-F antibodies than the anti-S from anti-human γ-globulin sera. Both rhesus and digested human γ globulins showed a stage of inhibition, when they completely prevented the precipitation of intact human γ globulin by its antiserum. Rhesus γ globulin, however, showed a reaction of partial identity (spur formation) on Ouchterlony analyses with human γ globulin and its antiserum, whereas digested human γ globulin showed a reaction of complete identity. The significance of these observations is discussed.