In Vitro Effect of Local Anesthetics on Candida albicans Germ Tube Formation

摘要

Objective: This study was planned to clarify the in vitro effect of lidocaine and bupivacaine on germ tube formation by Candida albicans isolates from cases of clinical vaginal candidiasis. Methods: Fourteen C. albicans strains (clinical vaginal isolates) were grown on Sabouraud agar for 24 h at 37℃ and tested as follows: 100 μl of a yeast suspension [10⁵ colony forming units (CFU)/ml of phosphate buffered saline (PBS)] was added to 500 μl of fresh human serum with lidocaine or bupivacaine (pure salts) in serial concentrations. The test was run in duplicate. Controls were prepared for each strain. After 4 h of incubation at 37℃, samples were taken from each vial and 200 yeasts were counted in a counting chamber. The pH of each suspension was measured. Results: The results are given as the mean of the 2 readings and are expressed as the percentage of blastoconidia with germ tubes/total blastoconidia. Conclusions: Our experiments show that both lidocaine and bupivacaine have a dose-dependent inhibitory effect, pH-independent, on germ tube formation by C. albicans and that both drugs seem to be promising in the treatment of genital candidiasis due to the combination of anesthetic and antifungal properties.