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CAMP Factor of Group B Streptococci: Production Assay and Neutralization by Sera from Immunized Rabbits and Experimentally Infected Cows

机译:B组链球菌的CAMP因子:免疫兔和实验感染牛的血清产生测定和中和

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摘要

Quantitative assay methods for CAMP factor activity of group B streptococci and for neutralization of this activity by animal sera are described. The course of the CAMP reaction is influenced by preincubation of red cells with staphylococcal beta-hemolysin and the rate of the reaction by the concentration of CAMP factor. The production of CAMP factor is enhanced by substitution of maltose for glucose in culture broth. Rising inhibitory titers to CAMP activity in sera of rabbits injected with sterile culture supernates of group B streptococci suggest that CAMP factor is an antigen in the rabbit. Cows with experimentally induced group B streptococcal mastitis also demonstrated significant rises in neutralizing titers for CAMP activity.
机译:描述了B组链球菌CAMP因子活性和动物血清中和该活性的定量测定方法。 CAMP反应的过程受红细胞与葡萄球菌β-溶血素的预孵育影响,反应速度受CAMP因子浓度的影响。通过用麦芽糖代替培养液中的葡萄糖来提高CAMP因子的产生。注射B组链球菌无菌培养上清液的家兔血清中CAMP活性的抑制效价升高表明CAMP因子是家兔中的一种抗原。实验诱导的B组链球菌性乳腺炎的母牛还表现出CAMP活性的中和效价显着升高。

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