Development and Validation of a New HPLC Method for the Determination of Gabapentin

摘要

A simple HPLC method was developed and validated for quantitation of gabapentin in pure form. The HPLC separation was achieved on a C18 5 μm Waters column (150 mm × 4.6 mm) using a mobile phase of methanol - potassium dihydrogen orthophosphate solution (20:80, v/v) containing 10% NaOH to adjust pH6.2 at a flow rate of 1.0 ml/min. The UV detector was operated at 275 nm. The method was validated for specificity, linearity, precision, accuracy, robustness and limit of quantitation. The degree of linearity of the calibration curves, the percent recoveries, limit of detection and quantitation for the HPLC method were determined. The method was found to be simple, specific, precise, accurate, and reproducible.