Background. In this study antimicrobial effect of ethanolic and aqueous extracts of Juglans regia bark in Iran was evaluated on four different oral bacteria, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, and Staphylococcus aureus. Methods. Aqueous and ethanol extracts of Juglans regia bark were prepared by using disk diffusion technique and Minimal Inhibitory Concentration (MIC) methods. Tetracycline 30 μg and Erythromycin 15 μg were used as positive control and water as negative control in disk diffusion and MIC methods. Data were analyzed by ANOVA test. Results. The results showed that S. sanguis and S. mutans were the most sensitive and the most resistant bacteria against ethanolic and aqueous extracts, respectively. Ethanolic extract had significant antibacterial effect against all tested bacteria. Aqueous extract did not show antibacterial effect on S. mutans, in contrast to ethanolic extract. Aqueous extract had significantly antibacterial effect against Staphylococcus aureus, S. salivarius, and S. sanguis compared to control (P < 0.0001), but it did not show effect on S. mutans when compared with Erythromycin. According to the obtained MIC values, ethanol extract of Juglans regia bark had the lowest rate. Conclusion. The results may provide the basis for using natural antimicrobial substance for oral hygiene prophylaxis purposes.
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机译：背景。在这项研究中，评估了伊朗胡桃皮的乙醇提取物和水提取物对四种不同的口腔细菌（变形链球菌，唾液链球菌，血链球菌和金黄色葡萄球菌）的抗菌作用。方法。采用圆盘扩散技术和最小抑菌浓度（MIC）法制备了胡桃皮的水和乙醇提取物。在圆盘扩散法和MIC法中，四环素30μg和红霉素15μg被用作阳性对照，水被用作阴性对照。通过ANOVA检验分析数据。结果。结果表明，桑氏葡萄球菌和变形链球菌分别是对乙醇提取物和水提取物最敏感和抵抗力最强的细菌。乙醇提取物对所有测试细菌均具有显着的抗菌作用。与乙醇提取物相比，水提取物对变形链球菌没有显示抗菌作用。水提取物对金黄色葡萄球菌，唾液链球菌和 S具有显着的抗菌作用。 sanguis 与对照（ P <0.0001）相比，但对 S没有显示作用。与红霉素比较时变种。根据获得的MIC值，胡桃皮的乙醇提取率最低。 结论。该结果可为使用天然抗菌物质预防口腔卫生提供依据。
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