Transcription factor YY1 is essential for iNKT cell development

摘要

Quantitative real-time PCR analysis of expression in various thymic subsets (DN (CD4 CD8 ), DP (CD4 CD8 ), CD4 (CD4 CD8 ), and CD8 (CD4 CD8 )) and at various thymic NKT cell developmental stages (S0 (CD1d-tet CD24 ), S1 (CD1d-tet CD24 CD44 NK1.1 ), S2 (CD1d-tet CD24 CD44 NK1.1 ), and S3 (CD1d-tet CD24 CD44 NK1.1 )) in C57BL/6 wild-type (WT) mice. Data are representative of two independent experiments. Frequency of NKT (CD1d-tet TCRβ ) cells in the thymus, spleen, and liver of WT and YY1-TKO mice. Cells were stained for CD4, CD8, TCRβ, and PBS57-loaded CD1d tetramer (CD1d-tet). Values indicate the percent of total mononuclear cells. Data are representative of five pairs of mice. Frequency and numbers of NKT cells in WT and YY1-TKO thymus, spleen, and liver. Each dot represents one mouse analyzed; *  P d Analysis of NKT cells at various developmental stages in the thymus of WT and YY1-TKO mice. Data shown are representative of five pairs of mice. Enumeration of NKT cells as described in ; NS not significant, *  P