机译
CPAP通过增强STAT3活性来促进血管生成和转移
摘要:CPAP overexpression contributes to tumor growth and metastasis in vivo. For orthotopic liver injection, GFP/Hep3B, or GFP-CPAP/Hep3B cells were injected into the left liver lobe of BALB/c nude mice. After 24 days of injection, the mice were sacrificed, and the liver tissues were collected for further analysis. Images of orthotopic liver xenograft tumors obtained from BALB/c nude mice injected with GFP/Hep3B ( = 7) or GFP-CPAP/Hep3B cells ( = 6) are shown (left); the tumor volume was calculated using the formula length × width × 0.5. Quantitative results are shown as the mean ± SEM. Student’s test, * = 0.0475. The liver tissues injected with GFP-CPAP/Hep3B but not GFP/Hep3B cells showed intrahepatic metastatic tumor growth. After 8 weeks of injection, the mouse liver and lung tissues from orthotopically injected mice were collected for H&E staining. The incidence of lung metastasis is shown. Seventy-two HCC specimens (T) and their adjacent normal tissues (NT) were collected to analyze the expression level of mRNA by RT-qPCR, which was presented as a ratio (T/NT). HCC tissues were divided into two size groups, small (≤2 cm, = 33) and large (≥5 cm, = 39). Mann–Whitney test, = 0.0473. RT-qPCR results were normalized to . GFP/Hep3B ( = 5) or GFP-CPAP/Hep3B ( = 5) cells were intra-splenically injected into BALB/c nude mice. After 8 weeks of injection, the mice were sacrificed, and the spleen, liver, and lung tissues were collected for analysis. Representative images of the spleen and liver tumors, H&E staining of the liver and lung tissues, and the incidence of liver and lung metastasis are shown. A representative image of H&E staining shows vascular invasion of the GFP-CPAP/Hep3B-derived liver tumor in the splenically injected mice. Arrows indicate vascular invasion of the GFP-CPAP/Hep3B cells. Scale bars, 100 μm