首页> 美国卫生研究院文献>British Journal of Pharmacology and Chemotherapy >Evidence for the involvement of a plasma kallikrein-kinin system in the immediate hypotension produced by endotoxin in anaesthetized rats.
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Evidence for the involvement of a plasma kallikrein-kinin system in the immediate hypotension produced by endotoxin in anaesthetized rats.

机译:血浆激肽释放酶激肽系统参与麻醉大鼠内毒素产生的即刻低血压的证据。

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摘要

1. In vitro incubation of normal rat plasma with endotoxin from E. coli (3-10 mg ml-1) in the incubation mixture) caused a dose-dependent increase in levels of free kinin and plasma kallikrein in the presence of o-phenanthroline, together with a mirror-image, dose-dependent decrease in the residual levels of the precursors, plasma prekallikrein and high-molecular-weight kininogen. Low-molecular-weight kininogen levels were not modified. 2. Intravenous injection of endotoxin (3-30 mg kg-1) into the femoral vein of anaesthetized rats resulted in dose-dependent hypotension. In blood collected up to 15 min after injection, the levels of prekallikrein and high-molecular-weight kininogen in plasma were decreased while levels of the active forms, plasma kallikrein and free kinin, showed a transient increase in the blood 1 min after administration of endotoxin. 3. A degradation product of bradykinin, des-Phe8-Arg9-bradykinin, as measured by a newly developed enzyme immunoassay, was detectable up to 5 min after administration of endotoxin. 4. Intravenous infusion of soybean trypsin inhibitor inhibited both the formation of bradykinin and des-Phe8-Arg9-bradykinin and the initial hypotension. 5. It can be concluded from our results that plasma prekallikrein is activated in the blood immediately after administration of endotoxin to rats and that bradykinin is a major cause of the immediate hypotension.
机译:1.正常大鼠血浆与大肠杆菌中的内毒素(3-10 mg ml-1)在孵育混合物中的体外孵育在邻菲咯啉存在下引起游离激肽和血浆激肽释放酶水平的剂量依赖性增加,以及前体,血浆前激肽释放酶和高分子量激肽原的残留水平的剂量依赖性下降的镜像关系。低分子量激肽原水平未改变。 2.将内毒素(3-30 mg kg-1)静脉注射到麻醉大鼠的股静脉中,导致剂量依赖性低血压。在注射后15分钟内收集的血液中,血浆中前激肽释放酶和高分子量激肽原原的水平降低,而活性形式血浆中激肽释放酶和游离激肽的水平在给药1分钟后血液中瞬时升高。内毒素。 3.用新开发的酶免疫法测定缓激肽的降解产物des-Phe8-Arg9-缓激肽,在施用内毒素后最多5分钟即可检测到。 4.静脉内注入大豆胰蛋白酶抑制剂可抑制缓激肽和des-Phe8-Arg9-缓激肽的形成,并抑制初始低血压。 5.从我们的结果可以得出结论,对大鼠施用内毒素后,血浆前激肽释放酶会立即在血液中被激活,而缓激肽是立即发生低血压的主要原因。

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