Antiproliferative and apoptotic effects of proteins from black seeds (Nigella sativa) on human breast MCF-7 cancer cell line

摘要

( ) Elution profile of gel filtration chromatography of protein extract seeds. The column HiLoad 16/600 Superdex 200 pg pre-equilibrated with 1X PBS buffer. The flow rate was 1 ml/min, and absorbance monitored at 280 nm. ( ) The electrophoretic profile on 4–20% Tris/glycine SDS PAGE gel. The samples analyzed crude seed proteins (C), gel filtration chromatography peaks (P1, P2, P3, and P4) and standard molecular weight ladder (L)