Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015–0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) and Pichia stipitis (0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching 26.9 ± 1.2 g/L and 86.5 ± 2.1% fermentation efficiency relative to the theoretical yield.
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机译：甜菜纸浆是甜菜加工的副产品，可用作第二代乙醇生产的原料。这项研究的目的是研究预处理，使用的纤维素酶和半纤维素酶制剂的剂量以及曝气对基于甜菜粕的同时糖化和发酵（SSF）过程中可发酵糖的释放和乙醇产量的影响。麦芽汁。对悬浮在2％w / w硫酸溶液中的甜菜果肉进行加压热预处理，其比例可提供12％的干物质。使用Viscozyme和Ultraflo Max（Novozymes）酶制剂（0.015–0.02 mL / g干物质）进行酶促水解。两种酵母菌株用于发酵：乙醇红（酿酒酵母）（1μg/ L）和致命毕赤酵母（0.5μg/ L），依次应用。结果表明，实现了甜菜果肉的高效同时糖化和发酵。在酶制剂的应用和接种乙醇红之间间隔6 h的酶促活化进一步改善了发酵性能，相对于理论产量，最高乙醇浓度达到26.9±1.2 g / L和86.5±2.1％发酵效率。
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