Hemifluorinated and fluorinated surfactants are lipophobic and, as such, non-detergent. Although they do not solubilize biological membranes, they can, after conventional solubilization, substitute for detergents to keep membrane proteins soluble, which generally improves their stability [Breyton, Chabaud, Chaudier, Pucci and Popot (2004) FEBS Lett. 564, 312–318]. In the present study, we show that (hemi)fluorinated surfactants can be used for in vitro synthesis of membrane proteins: they do not interfere with protein synthesis, and they provide a suitable environment for MscL, a pentameric mechanosensitive channel, to fold and oligomerize to its native functional state. Following synthesis, both types of surfactants can be used to deliver MscL directly to pre-formed lipid vesicles. The electrophysiological activity of MscL synthesized in vitro in the presence of either hemi- or per-fluorinated surfactant is similar to that of the protein expressed in vivo.
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机译：半氟化和氟化表面活性剂是疏脂的，因此是非洗涤剂的。尽管它们不溶解生物膜，但是在常规的溶解之后，它们可以代替去污剂以保持膜蛋白可溶，这通常会提高其稳定性[Breyton，Chabaud，Chaudier，Pucci and Popot（2004）FEBS Lett。 564 ，312–318]。在本研究中，我们表明（半）氟化表面活性剂可用于膜蛋白的体外合成：它们不干扰蛋白合成，并且它们为五聚体机械敏感性通道MscL提供了合适的折叠和寡聚环境。到其原始功能状态。合成后，两种类型的表面活性剂均可用于将MscL直接递送至预先形成的脂质囊泡。在存在半氟化或全氟化表面活性剂的情况下，体外合成的MscL的电生理活性与体内表达的蛋白质相似。
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