首页> 美国卫生研究院文献>The American Journal of Pathology >Brain microvascular smooth muscle and endothelial cells produce granulocyte macrophage colony-stimulating factor and support colony formation of granulocyte-macrophage-like cells.
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Brain microvascular smooth muscle and endothelial cells produce granulocyte macrophage colony-stimulating factor and support colony formation of granulocyte-macrophage-like cells.

机译:脑微血管平滑肌和内皮细胞产生粒细胞巨噬细胞集落刺激因子并支持粒细胞-巨噬细胞样细胞的集落形成。

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摘要

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent stimulator of macrophages and neutrophils and plays a role in inflammatory diseases. In this article, we report that mouse brain-derived microvascular smooth muscle cells (SM) and endothelial cells (En) in coculture with splenocytes support the colony proliferation of immature granulocyte-macrophage-like (GM) cells. Unstimulated SM and En cells release GM-CSF as shown by ELISA assay and SM expresses mRNA for GM-CSF by polymerase chain reaction (PCR). Stimulation of SM and En by a nonspecific activator (lipopolysaccharide) results in upregulation of GM-CSF production. GM colonies cannot be grown on cultured astrocytes or on extracellular matrix alone prepared from smooth muscle or endothelium. However, colonies form on the extracellular matrix and on astrocytes, either in the presence of SM- or En-conditioned medium or after the addition of recombinant GM-CSF. The GM cells are positive for nonspecific esterase, peroxidase, and MAC-1 markers but are negative for FC gamma receptors and for Thy 1.2, CD8, CD4, MHC class II, and Asialo GM1 markers. These observations emphasize the possibility for active participation of brain microvasculature SM and En in acute inflammatory reactions of the central nervous system.
机译:粒细胞-巨噬细胞集落刺激因子(GM-CSF)是巨噬细胞和嗜中性粒细胞的有效刺激剂,在炎症性疾病中起作用。在这篇文章中,我们报告与脾细胞共培养的小鼠脑源性微血管平滑肌细胞(SM)和内皮细胞(En)支持未成熟的粒细胞-巨噬细胞样(GM)细胞的集落增殖。如ELISA测定所示,未刺激的SM和En细胞释放GM-CSF,并且SM通过聚合酶链反应(PCR)表达GM-CSF的mRNA。非特异性激活剂(脂多糖)刺激SM和En导致GM-CSF产生上调。 GM集落不能在培养的星形胶质细胞或仅由平滑肌或内皮制备的细胞外基质上生长。但是,在SM或En条件培养基的存在下或添加重组GM-CSF后,集落会在细胞外基质和星形胶质细胞上形成。 GM细胞对非特异性酯酶,过氧化物酶和MAC-1标记呈阳性,但对FCγ受体和Thy 1.2,CD8,CD4,MHC II类和Asialo GM1标记呈阴性。这些观察结果强调了大脑微脉管系统SM和En积极参与中枢神经系统的急性炎症反应的可能性。

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