Crystallization and X-ray data collection of HP0902 from Helicobacter pylori 26695

摘要

HP0902 from Helicobacter pylori 26695 belongs to the cupin superfamily of proteins, which encompasses proteins with a great diversity in function. In this work, two types of recombinant HP0902 protein were crystallized: one with an N-terminal His6 tag (^H6HP0902) and the other with a C-terminal His6 tag (HP0902^H6). The ^H6HP0902 crystal diffracted to 1.40 Å resolution and belonged to space group P21, with unit-cell parameters a = 33.5, b = 78.6, c = 41.4 Å. The HP0902^H6 crystal belonged to space group P43212 or P41212 and diffracted to 2.5 Å resolution, with unit-cell parameters a = b = 50.4, c = 142.0 Å.