Crystallographic analysis of RsmA a ribosomal RNA small subunit methyltransferase A from Staphylococcus aureus

摘要

RsmA, a ribosomal RNA small subunit methyltransferase from Staphylococcus aureus, catalyzes the N ⁶ methylation of adenine in 16S rRNA. In this study, RsmA from Staphylococcus aureus was cloned, expressed, purified and crystallized. The crystal belonged to space group C2, with unit-cell parameters a = 84.38, b = 157.76, c = 96.50 Å, β = 95.04°. X-ray diffraction data were collected to a resolution of 3.2 Å. The self-rotation function and the Matthews coefficient suggested the presence of two molecules in the asymmetric unit.