布氏田鼠冷暴露中褐色脂肪组织的增补及解偶联蛋白基因表达

摘要

布氏田鼠(Microtus brandti)随机分组暴露在冷环境［12L∶12D，(4±2)℃］中12?h，1 d，3 d，7 d，14 d，21 d和28 d；对照组生活在温暖环境下［12L∶12D，(25±2)℃］。与对照组相比，布氏田鼠的褐色脂肪组织(BAT)重量在冷暴露12?h～3?d时降低，7～21?d时则增加。7～28?d冷暴露组动物的BAT总蛋白和总DNA含量均比对照组明显提高。冷环境中的布氏田鼠解偶联蛋白(UCP)的mRNA随时间的延长而表达上调，在冷暴露21?d时达到高峰。结果表明，冷暴露能够诱导布氏田鼠BAT细胞增补和UCP基因表达，从而使适应性产热增加。%Brandt'　s voles (Microtus brandti) were randomly divided into seven groups and exposed to cold temperature［12L∶12D，(4±2)℃］ for 12 hours，1，3 ，7，14，21 and 28 days respectively；the control group was kept in warm place ［12L∶12D，(25±2)℃］. Compared with the control，the weight of BAT and the total contents of DNA in BAT decreased during cold exposure from 12 hours to 3 days，but increased significantly from 7 to 28 days. Cold exposure also induced the increase of protein contents of BAT. In molecular level，the contents of UCP mRNA in BAT increased significantly and reached peak at 21 days. The results suggested that cold exposure could induce the recruitment of BAT cell and the expression of UCP gene，resulting in the increase of adaptive thermogenesis in Brandt'　s voles.