目的 观察不同制剂工艺生产的两种剂型银杏叶提取物 (EGB)对神经元缺氧/复氧损伤的保护作用,探讨EGB在缺血性脑血管疾病中的应用价值,寻求最佳给药途径.方法 原代培养新生SD大鼠皮层神经元,建立缺氧/复氧损伤模型.将细胞分为以下6组:正常培养组、缺氧/复氧组、EGB761低剂量组、EGB761高剂量组、EGB1212低剂量组和EGB1212高剂量组.后4组分别在神经损伤前24h给予相应药物处理,采用MTT法测定细胞存活率,乳酸脱氢酶(LDH)释放测定检测细胞毒作用,并观察神经元形态变化.结果 缺氧/复氧损伤造成神经元细胞形态的改变,表现为轴突断裂、核固缩、细胞水肿.EGB761和EGB1212预处理可减轻神经元形态学改变.EGB761及EGB1212干预均能提高细胞存活率,减少LDH释放,且呈剂量依赖性.同等剂量下EGB1212对神经元的保护作用优于EGB761(P＜0.05).结论 EGB对缺氧/复氧造成的神经元损害有保护作用,新工艺剂型EGB1212比传统剂型EGB761保护作用更为突出.%Objective To investigate the protective effect of extracts of gingko biloba (EGB) on rat neuron during hypoxi-a/reoxygenation (H/R). Methods The cortical nerve cells were cultured in vitro for 10d and randomly divided into 6 groups: the control group, H/R group, EGB761 low-dose group, EGB761 high-dose group, EGB1212 low-dose group and EGB1212 high- dose group. In EGB- treated groups EGB761(0.01 and 0.lmg/L) or EGB1212(0.01 and O.lmg/L) were added in culture media 24h before H/R, respectively. The morphology of neurons was observed with microscopy, neuronal survival rate was examined with MTT assay and LDH was measured with LDH release assay in the culture media collected after hypoxia for 2.5h and reoxy-genation for 24h. Results The neuronal cell was swelling after 2.5h hypoxia and 24h reoxygenation insult. MTT assay suggested the neuronal survival rate deceased to 50% compared to normal condition. LDH level in the H/R group was increased as compared with the control group. In all EGB- treated groups, the survival rate of the neurons was increased and the LDH levels were decreased as compared with the H/R group. The protective effects ofEGB1212 were more prominent than those of EGB761 at the same concentration. Conclusion H/R can induce the damage of neurons in rats and EGB may protect neurons against the insults with a dose dependent manner.
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