神经生长因子联合溴隐亭对垂体GH3细胞的抑制作用

摘要

Objective To investigate the effects of nerve growth factor (NGF) and bromocriptine (BC) on the proliferation of pituitary adenoma GH3 cells in vitro.Methods In serum- free culture conditions, GH3 cells were divided into 3 groups: control, bromocriptine and NGF + bromocriptine.The effects on proliferation of GH3 cells were tested by CCK- 8 assay.Results Compared with the control group, bromocriptine alone had no inhibition effects on proliferation of GH3 cells (P＞0.05).However, proliferation of GH3 cells was effectively inhibited by bromocriptine on d2, d4, d6 after NGF treatment (P＜0.01).Conclusion NGF can enhance the inhibition effects of bromocriptine on proliferation of pituitary adenoma GH3 cell in vitro.%目的 探讨神经生长因子(NGF)联合溴隐亭对垂体腺瘤GH3细胞增殖的影响.方法 在无血清的培养条件下,将GH3细胞分成3组:对照组、溴隐亭组、NGF+溴隐亭组,采用CCK-8法检测不同药物干预后GH3细胞的成活率.结果 与对照组比较,NGF干预后GH3细胞的形态无明显改变.单独溴隐亭治疗对GH3细胞增殖的抑制作用不明显,而NGF干预后2、4、6d分别再给予溴隐亭治疗则对GH3细胞增殖均有明显抑制作用(P＜0.01),其中NGF干预后4d及6d时溴隐亭的抑制效果更明显.结论 NGF能促进溴隐亭对GH3细胞增殖的抑制作用.