应用SELDI-TOF-MS技术研究急性胰腺炎特异性血清标志物

摘要

目的 应用蛋白芯片表面加强激光解析电离-飞行时间-质谱(SELDI-TOF-MS)术揭示胰腺炎患者血清蛋白指纹特征,查找病情特异性血清标志物,探讨急性重症胰腺炎的早期诊断及评估的新方法.方法 收集急性胰腺炎患者血清标本52份,其中急性重症胰腺炎患者24份(SAP组),急性轻型胰腺炎28份(MAP组),同期健康人群血清52份(正常对照组),应用SELDI检测急性胰腺炎患者及健康人群的蛋白质谱,按照临床胰腺炎分级,获得不同临床级别胰腺炎和健康人群的血清蛋白指纹图谱,应用计算机软件分析处理所有峰谱.结果 在质荷比(M/Z)1 000~20 000检出到127个蛋白峰,其中MAP组与正常对照组比较5个峰差异有明显统计学意义(P＜0.05),MAP组与SAP组比较3个峰值差异有统计学意义(P＜0.05).结论 用SELDI-TOF-MS蛋白指纹图谱技术可筛选出有意义的生物标记差异蛋白,这对急性胰腺炎的诊断以及病情轻重的评估提供了新的路径.%Objective To analyze the serum proteomic pattern in patients with acute pancreatitis by using surface- enhanced laser desorption/ionization time- of- flight mass spectrometry (SELDI- TOF- MS).Methods Serum samples were collected from 52 patients with acute pancreatitis, including 24 cases of severe acute pancreatitis (SAP), 28 cases of mild acute pancreatitis (MAP); 52 serum samples from healthy subjects were used as controls.The proteomic patterns of all serum samples were analyzed by SELDI- TOF- MS method.Results Five protein peaks were found related to acute pancreatitis, and there were three different protein peaks between MAP and SAP.Conclusion Proteomic technology may identify novel biomarkers in serum of patients with pancreatitis.