miR-340在膀胱尿路上皮癌中的表达及其靶基因预测的生物信息学分析

摘要

Objective To investigate the expression of miR-340 in bladder urothelial carcinoma,and to analyze the target genes of miR-340 with bioinformatics.Methods miRNA array was applied to detect miRNA expression profile in bladder urothelial carcinoma,and qRT-PCR was used for validation.The bioinformatic analysis of the target genes of miR-340,the involved gene ontology and signal transduction pathway was performed.Results The expression of miR340 significantly decreased in bladder urothelial cancer compared with that normal bladder urothelial tissues.The functions of predicted target genes of miR-340 were enriched in cell adhesion,biological adhesion and cell-cell adhesion.KEGG pathway analysis showed the the predicted target genes were involved in pathways of cancer and cell cycle.Conclusion The expression of miR-340 significantly decreases in bladder urothelial carcinoma;and the predicted target genes of miR-340 are significantly enriched in tumor related signaling pathways.%目的 探讨miR-340在膀胱尿路上皮癌中的表达,并对其预测的靶基因进行生物信息学分析,为miR-340在膀胱尿路上皮癌发生中的作用机制研究提供理论基础.方法 采用miRNA芯片技术检测膀胱尿路上皮癌及正常膀胱黏膜组织中miRNA表达谱,用实时定量PCR法进行验证,挑选具有显著表达差异的miR-340为进一步研究对象,利用生物信息学方法,对miR-340的靶基因进行预测,并对其靶基因进行基因本体(GO)功能富集分析及KEGG信号转导通路富集分析.结果 miRNA表达谱芯片、实时定量PCR法均提示miR-340在膀胱尿路上皮癌中较癌旁正常膀胱组织中表达明显降低.miR-340预测靶基因集合功能富集于细胞黏附(cell adhesion)、生物黏附(biological adhesion)和细胞间黏附(cell-cell adhesion)等,KEGG通路分析涉及癌通路(pathways in cancer)和细胞周期通路(pathways in cell cycle)等.结论 miR-340在膀胱尿路上皮癌中呈低表达,miR-340预测靶基因集合显著富集在与肿瘤发生相关的信号通路中.