复方藤梨根制剂抑制小鼠结肠癌人工转移实验研究

摘要

Objective To investigate the effect of Fufang Tengligen mixture on pathophysiology, gross anatomy, molecular imaging, and pathology of the artificial blood metastasis from colon cancer in mice. Methods Fifty BALB/c mice were randomly divided into 5 groups:Fufang Tengligen high- , middle-, and low-dose groups(2.24g/10g, 1.12 g/10g, and 0.56g/10 g,respectively), model group, and control group, 10 mice in each group. Except control group, other groups were intravenously injected with murine colon adenocarcinoma cells of 0.1 mL (4í106/mL) to make the artificial blood metastasis model.All mice in Fufang Tengligen mixture groups were given intra-gastrically with 0.2 mL/10 g medicine for 21 consecutive days. PET scanning was performed on day 1, 7, 14, and 21 after modeling. The T/N ratio, average SUV value of computer generated PET-CT images of the tumor were cal-culated for quantitive analysis. All mice were sacrificed on day 21 to perform lung biopsy. Results The symptom of cachexia was more obvious in model and Fufang Tengligen low-dose group than that in Fufang Tengligen high-and midddle-dose groups. The T/N ratio and SUV value were increased in tumor bearing groups than those in control group(P<0.01).Compared with model group, the T/N ratio and SUV value of the images of the tumor and non-tumor area were lower in Fufang Tengligen groups(P<0.01); the values were lower in Fufang Tengligen high-dose group than those in the middle-and low-dose groups(P<0.05, P<0.01). Pathological examination showed metas-tasis in both lungs of tumor bearing groups, while no metastasis in brain, liver, and kidney was seen as well as precancerous lesions. Conclusion Fufang Tengligen mixture may have anti-tumor effect on lung cancer from colon cancer in mice in a dose-dependent manner.%目的：观察复方藤梨根制剂对小鼠结肠癌人工转移模型的病理生理、大体解剖、分子影像及病理的影响。方法 BALB/c小鼠50只，随机分成复方藤梨根高、中、低剂量组、模型组、空白对照组5组，每组10只。除空白对照组外均尾静脉注射C26结肠腺癌细胞单细胞悬液（密度：4×106/mL）0.1mL，形成结肠癌人工血行转移模型。按照小鼠灌胃容积0.2mL/10g分别予复方藤梨根制剂2.24g/10g、1.12g/10g、0.56g/10g、生理盐水灌胃。连续灌胃21天，第1、7、14、21天观察各组小鼠一般情况，异氟烷吸入式麻醉后，进行PET扫描，通过PET图像、靶区和非靶区比值（T/N）、标准化摄取值比值（SUV）数据分析，观察各组小鼠肿瘤生长情况。第21天处死小鼠，做肺组织病理活检。结果①模型组和复方藤梨根低剂量组小鼠恶液质症状较高、中剂量组小鼠明显；②各荷瘤组T/N值和SUV值较空白对照组明显升高（P<0.01），复方藤梨根制剂各组和模型组比较，肿瘤和非肿瘤区域T/N和SUV比值较低（P<0.01），高剂量组低于中、低剂量组，差异有统计学意义（P<0.05，P<0.01）；③病理显示，各荷瘤组两肺均有转移灶，脑、肝脏、肾脏均未见明显肿瘤转移灶和癌前病变。结论经尾静脉接种C26结肠腺癌细胞可在Balb/c小鼠上形成稳定的肺转移模型，复方藤梨根制剂可以减少小鼠结肠癌人工肺转移模型的肿瘤活性，并抑制转移，有一定剂量依赖性。