蛋氨酸脑啡肽对脐血来源CIK细胞作用的探索

摘要

目的：尝试采用蛋氨酸脑啡肽（MENK）增强脐血来源的细胞因子诱导杀伤细胞（CIK）的增殖能力和肿瘤细胞杀伤能力，探索如何有效缩短细胞治疗周期并增强其疗效。方法以原有细胞因子配方联合MENK作为观察组，以原有配方为对照组，通过细胞计数和细胞杀伤实验检测MENK对脐血CIK细胞的作用。结果在细胞培养的第9天、第12天，观察组CIK细胞数量分别为（12.1±1.9）×109和（17.6±2.2）×109，高于同期对照组（9.4±1.3）×109和（10.7±1.7）×109；在肿瘤细胞杀伤试验中，观察组CIK的杀伤率在第9天、第12天分别为（49.82±1.86）%和（60.66±2.07）%，同期对照组杀伤率为（30.46±1.69）%和（50.46±1.94）%，观察组与对照组差异有统计学意义（P<0.05）。结论在细胞因子诱导脐血来源单个核细胞成为杀伤细胞的过程中，MENK具有促进杀伤细胞增殖，增强肿瘤细胞杀伤能力的作用；可使脐血来源CIK提前达到回输标准，缩短治疗周期。%ObjectiveTo observe whether the cytotherapy period was cut or not,and the effect of proliferation and cytotoxicity of cytokine induced killer cells(CIK)derived from cord blood by application of Methionine Enkephalin(MENK).Method All mononuclear cells from cord blood were divided into 2 groups,the control group cultivated with original cytokine formula,while the test group with the combination of MENK and the original. The effect was investigated by cell counting and cytotoxicity test.Result Both of theResultsin proliferation and cytotoxicity test were signifi cantly higher in test group than in control group(P<0.05). The cells count showed(12.1±1.9)×109 and(17.6±2.2)×109 in test group at Day 9 and Day 12 respectively during cultivation,more than(9.4±1.3)×109 and(10.7±1.7)×109 in control. While the cytotoxicity rate of CIK revealed(49.82±1.86)% and(60.66±2.07)% in test group,comparing with(30.46±1.69)% and(50.46±1.94)% in control.ConclusionIt was illustrated that the capability of proliferation and cytotoxicity promoted in CIK derived from mononuclear cells in cord blood by utilizing MENK and the cytotherapy period shortened as well.