胃癌组织MGMT蛋白的表达及临床意义

摘要

Objective This study aimed to investigate and to assess the relationship between the expression of MGMT protein in gastric cancer tissues,biological behavior of gastric cancer and long-term prognosis. Methods A total of 262 cases of gastric cancer tissues and normal tissues adjacent to carcinomas were collected. The expressions of intra-tissue MGMT proteins were measured using immunohistochemistry. The difference of expression of MGMT proteins between gastric cancer tissues and normal tissues adjacent to carcinomas,the relationship between expression of MGMT proteins in gastric cancer tissues and clinicopathological parameter of gastric cancer,and the relationship between expression of MGMT proteins in gastric cancer tissues and 5-year mortality and overall survival of patients with gastric cancers were analyzed statistically. Results Positive rate of expression of MGMT proteins was markedly lower in gastric cancer tissues than in normal tissues adjacent to carcinomas. The expression of MGMT protein in gastric cancer tissues was obviously associated with tumor size,invasion depth,lymph node metastasis,distant metastasis,peritoneal dissemination,and TNM stage. The negative expression of MGMT protein in gastric cancer tissues was identified as an independent risk factor for 5-year mortality and overall survival of gastric cancers. Conclusion The expression of MGMT protein in gastric cancer tissues is decreased markedly. Low expression of MGMT protein in gastric cancer tissues is highly associated with malignant biological behavior of gastric cancer,and independently related to long-term poor prognosis of gastric cancer.%目的：检测胃癌组织MGMT蛋白表达情况，分析其与胃癌生物学行为及长期预后的相关性。方法收集262例胃癌组织及癌旁正常组织，采用免疫组织化学法测定组织内MGMT蛋白的表达情况，统计分析胃癌组织及癌旁正常组织MGMT蛋白表达的差异、胃癌组织MGMT蛋白表达与胃癌临床病理参数的关系及胃癌组织MGMT蛋白表达与胃癌患者5年死亡及生存时间的关系。结果胃癌组织MGMT蛋白表达阳性率显著低于癌旁正常组织，胃癌组织MGMT蛋白表达与胃癌肿瘤大小、浸润深度分级、淋巴结转移分级、远处转移分级、腹膜扩散情况和TNM分期相关，胃癌组织MGMT蛋白阴性表达是胃癌5年死亡及5年生存时间的独立危险因素。结论胃癌组织MGMT蛋白表达显著降低，胃癌组织MGMT蛋白低表达与胃癌恶性生物学行为密切相关，与胃癌长期不良预后独立相关。