Objective:to different standard curve fitting model for chemiluminescence immune analysis analysis of the determination results. Method:Determination of thyroxine standard with chemiluminescence immunoassay method, result of spline function, four parameters, three polynomial and logarithmic functions of four kinds of model fitting the standard curve, analyzes the effects of fitting model on the results of determination. Results: the determination of serum thyroid hormone function fitting method with four parameters model as the best fitting model, there is great difference compared with the other models. Conclusion:the determination of chemiluminescence immunoassay method should be chosen according to different standard curve fitting model of different test items, and provide accurate and reliable results.% 目的对不同标准曲线拟合模式对化学发光免疫分析测定结果的影响进行分析探讨。方法用化学发光免疫分析法对甲状腺素标准品进行测定,结果选用样条函数、四参数、三次多项式和对数函数等四种模式拟合其标准曲线,分析各拟合模式对测定结果的影响。结果测定血清甲状腺素时曲线拟合方法中以四参数拟合模式为最佳拟合模式,与其他模式比较有较大的差异。结论化学发光免疫法测定时应根据不同检测项目选择不同标准曲线拟合模式,提供准确可靠的结果。
展开▼