AIM: To evaluate the effects of gliadin on the oxidative environment in the'in vivo-like' model of a three-dimensional cell culture system.METHODS: LoVo cell line (intestinal adenocarcinoma)multicellular spheroids were treated with digested gliadin (with albumin used as a control). Spheroid volumes, cell viability and morphology, lactate dehydrogenase (LDH)release, content of reduced glutathione (GSH) and activity of GSH-related enzymes were examined. The data were statistically analyzed using the Student's t-test (P＜0.05).was considered statistically significant.RESULTS: Gliadin reduced cell viability (from 20% to 60%)and led to morphological alterations characterized by apoptotic findings and cytoskeletal injuries. LDH activity increased. The content of GSH reduced (-20% vs controls),and activity of GSH-related enzymes was significantly inhibited.CONCLUSION: Gliadin treatment induces an imbalance in the antioxidative mechanism of cells cultured by the three-dimensional technique. This alteration may explain the cell damage directly caused by gliadin and the subsequent morphological abnormalities.
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