Protective effects of Asian green vegetables against oxidant induced cytotoxicity

摘要

AIM: To evaluate the antioxidant and phase Ⅱ detoxification enzyme inducing ability of green leaf vegetables consumed in Asia.METHODS: The antioxidant properties of six commonly consumed Asian vegetables were determined using the ABTS, DPPH, deoxyribose, PR bleaching and ironascorbate induced lipid peroxidation assay. Induce of phase Ⅱ detoxification enzymes was also determined for each respective vegetable extract. Protection against authentic ONOO- and HOCI mediated cytotoxicity in human colon HCT116 cells was determined using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide) viability assay.RESULTS: All of the extracts derived from green leaf vegetables exhibited antioxidant properties, while also having cytoprotective effects against ONOO- and HOCI mediated cytotoxicity. In addition, evaluation of the phase Ⅱ enzyme inducing ability of each extract,as assessed by quinone reductase and glutathioneS-transferase activities, showed significant variation between the vegetables analyzed.CONCLUSION: Green leaf vegetables are potential sources of antioxidants and phase Ⅱ detoxification enzyme inducers in the Asian diet. It is likely that consumption of such vegetables is a major source of beneficial phytochemical constituents that may protect against colonic damage.