即时灸对寒湿凝滞型痛经大鼠子宫组织前列腺素及加压素含量的影响

摘要

Objective To observe the effect of instant moxibustion on the levels of prostaglandin E2 (PGE2),prostaglandin F2α (PGE2α) and arginine vasopressin (AVP) in the uterine tissues of dismenorrhea rats with cold-damp congealing and stagnation type and to explore its possible mechanism.Methods Female Wistar rats were randomly divided into blank group,model group,herble medicine group,pre-moxibustion group and instant moxibustion group,with 9 rats in each group.Cold-damp congealing and stagnation type primary dismenorrhea models were established by adopting (0±1)℃ ice waterextraction method combined with estradiol benzoate injection method.After modeling on the 8th day,in herble medicine group,Tongjingbao granules was given to the rats by intragastric administration.In pre-moxibustion group,mild moxibustion was carried out at "Shénquè"(神阙 CV 8) and “Guānyuán"(关CV 4) of the rats for 20 min at each acupoint.In instant moxibustion group,moxibustion as that in pre-moxibustion group was conducted for once after injection with oxytocin on the 11th day.ELISA was adopted to detect the levels of PGE2 and PGE2α in the uterine tissues of rats,and radioimmunoassay was used for detection of AVP level in the uterine tissues of rats.Results Compared with the model group,the latent period of rats in herbal medicine group,premoxibustion group and instant moxibustion group obviously prolonged,the number of times of torsion reduced,and the total score of torsion decreased (P＜0.01);compared with herbal medicine group,the latent period of rats in instant moxibustion group obviously prolonged,and the total score of torsion decreased (P＜0.05 or P＜0.01);compared with pre-moxibustion group,the number of times of torsion of rats in instant moxibustion group reduced,and the total score of torsion decreased (P＜0.01).Compared with blank group,the levels of PGE2α and AVP and the ratio of PGE2α and PGE2 in the uterine tissues of rats in model group significantly increased (P＜0.01),and the PGE2 level significantly reduced (P＜0.01);compared with model group,the PGE2α level and the ratio of PGE2α and PGE2 in the uterine tissues of rats in herble medicine group,pre-moxibustion group and instant moxibustion group obviously reduced (P＜0.05 or P＜0.01),the PGE2 level obviously increased (P＜0.01),and the AVP level in the uterine tissues of rats in pre-moxibustion group and instant moxibustion group obviously reduced (P＜0.05 or P＜0.01);compared with herbal medicine group,the levels of PGE2α and AVP and the ratio of PGE2α and PGE2 in the uterine tissues of rats in instant moxibustion group significantly reduced (P＜0.05 or P＜0.01);compared with pre-moxibustion group,the PGE2α level and the ratio of PGE2α and PGE2 in the uterine tissues of rats in instant moxibustion group obviously reduced (P＜0.05),and the PGE2 level obviously increased (P＜0.01).Conclusion Both pre-moxibustion and instant moxibustion can obviously inhibit spasmodic uterine smooth muscle contraction of rats with dismenorrhea,regulate imbalanced levels of PGE2α and PGE2,reduce the AVP level,so as to improve the uterine hypoxia-ischemia,and play a role in alleviating pain.The efficacy of instant moxibustion was superior to that of pre-moxibustion.%目的:观察即时艾灸对寒湿凝滞型痛经大鼠子宫组织前列腺素E2(PGE2)、前列腺素F2α(PGE2α)及加压素(AVP)含量的影响.方法:雌性Wistar大鼠随机分为空白组、模型组、痛经宝组、预先灸组、即刻灸组,每组9只.采用(0±1)℃冰水浸泡法结合苯甲酸雌二醇注射法复制寒湿凝滞型原发性痛经模型.痛经宝组于第8天造模后,予痛经宝颗粒灌胃给药.预先灸组于第8天造模后,温和灸“神阙” “关元”穴,每穴10 min.即刻灸组于第1 1天注射缩宫素后同法施灸1次.采用酶联免疫法检测大鼠子宫组织PGE2、PGE2α含量,放射免疫分析法检测大鼠子宫组织AVP含量.结果:与模型组比较,痛经宝组、预先灸组、即刻灸组大鼠的潜伏期明显延长、扭体次数均减少、扭体总分均降低(均P＜0.01);与痛经宝组比较,即刻灸组大鼠的潜伏期明显延长、扭体总分均降低(P＜0.05,P＜0.01);与预先灸组比较,即刻灸组大鼠扭体次数均减少、扭体总分均降低(均P＜0.01).与空白组比较,模型组大鼠子宫组织PGE2α、AVP含量及P GE2α/PGE2比值均显著升高(均P＜0.01),PGE2含量显著降低(P＜0.01);与模型组比较,痛经宝组、预先灸组、即刻灸组大鼠子宫组织PGE2α含量及PGE2α/PGE2均明显降低(均P＜0.05,P＜0.01),PGE2含量均明显升高(均P＜0.01),预先灸组与即刻灸组大鼠子宫组织AVP含量均明显降低(P＜0.05,P＜0.01);与痛经宝组比较,即刻灸组大鼠子宫组织PGE2α、AVP含量及PGE2α/PGE2均明显降低(P＜0.05,P＜0.01);与预先灸组比较,即刻灸组大鼠子宫组织PGE2α及PGE2α/PGE2均明显降低(均P＜0.05),PGE2含量均明显升高(均P＜0.01).结论:预先灸与即刻灸均可明显抑制痛经大鼠子宫平滑肌痉挛性收缩,调节失衡的PGE2α和PGE2,降低AVP的含量,进而改善子宫局部缺血缺氧状态,起到镇痛作用,但即刻灸疗效优于预先灸.