酸脂清胶囊对尿酸性肾病大鼠肾功能及 TNF -α的影响

摘要

Objective:To observe the effect and explore the mechanism of Suanzhiqing capsule on renal function and TNF -αlevel in Ne-phropathy Rats with Uric Acid .Methods: Male SD rats were divided into five groups including normal group , model group , sodium chloride group , suanzhiqing capsule treatment group , and allopurinol treatment group .Nephropathy rat with uric acid were established by giving adenine and ethambuto .Then model rats were administered intragastrically with sodium chloride , suanzhiqing capsule , or allo-purinol for three weeks.The blood urea nitrogen (BUN), creatinine (Cr), uric acid (UA) in blood and tumor necrosis factor alpha (TNF-α) in renal tissue were determined.Results:(1) Compared with the normal group, the levels of BUN.Cr, and UA in blood in model group significantly increased (P<0.05).(2) Compared with the sodium chloride group , the levels of BUN, and UA in blood in suanzhiqing capsule treatment group and allopurinol treatment group significantly decreased (P<0.05).Although the levels of Cr in al-lopurinol treated group decreased , only in suanzhiqing capsule treated group were significantly .(3)Comparing the levels of BUN.Cr, and UA in suanzhiqing capsule treatment group with those in allopurinol treatment group , there was no significant difference between the two groups (P>0.05).(4) The optical density value of TNF-αin renal tissue of the sodium chloride group was significantly higher than that in normal group(P<0.05).The optical density value of TNF-αin renal tissue of suanzhiqing capsule treatment group and allopurinol treatment group were significantly lower than that in sodium chloride group (P<0.05).(5) Compared with the model group, uratic crystallization and inflammatory cell infiltration were significantly reduced , and tubular expansion had been relieved in both suanzhiqing capsule treatment group and allopurinol treatment group .Conclusion:Suanzhiqing capsule could reduce the levels of BUN .Cr, and UA in blood of nephropathy rats with uric acid , decrease uratic crystallization and inflammatory cell infiltration , and lower TNF-αlevel in re-nal tissue, thus it could have a beneficial effect on uric acid nephropathy rats .%目的：观察酸脂清胶囊对尿酸性肾病大鼠肾功能及TNF-α的影响，探讨其作用的机理。方法：将6周龄SD雄性大鼠分为5组，分别为正常组、模型组、生理盐水组、酸脂清治疗组、别嘌醇治疗组。后4组用腺嘌呤、乙胺丁醇造模，成功后分别给予生理盐水、酸脂清、别嘌醇灌胃给药，3周后检测各组大鼠血中尿素氮（BUN）、肌酐（Cr）、尿酸（UA），同时用酶联反应法（Elisa法）测量各组肾脏组织中肿瘤坏死因子α（ TNF-α）的含量。结果：1）造模2周后，与正常组比较，模型组大鼠血中BUN、Cr、UA均明显升高（P＜0.05），证实造模成功。2）治疗3周后，与生理盐水组相比，酸脂清治疗组、别嘌醇治疗组大鼠血中BUN、UA均明显下降（P＜0.05），酸脂清治疗组和别嘌醇治疗组Cr均有下降，但只有酸脂清治疗组有统计学意义（P＜0.05）。3）酸脂清治疗组和别嘌醇治疗组血中BUN、Cr、UA相比，差异无统计学意义（P＞0.05）。4）Elisa法测定各组TNF-α的浓度，与正常组相比，生理盐水组TNF-α明显升高（ P＜0.05）；与生理盐水组相比，酸脂清治疗组、别嘌醇治疗组TNF-α含量明显减少（P＜0.05）；5）肾组织病理切片显示：与模型组相比，酸脂清治疗组、别嘌醇治疗组尿酸盐结晶沉积、炎性细胞浸润均明显减少，肾小管扩张改善。结论：酸脂清胶囊能够降低尿酸性肾病大鼠血中BUN、Cr、UA的含量，减少尿酸盐结晶在肾小管中沉积及炎性细胞浸润，降低TNF-α在肾组织中的含量，从而达到治疗的作用。